What is the difference between DNaseI and DnaseII enzymes?
DNase I is an endonuclease that nonspecifically cleaves DNA, preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, to release di-, tri- and oligonucleotide products with 5´ -phosphorylated and 3´ -hydroxylated ends. DNase I acts on single- and double-stranded DNA, chromatin and RNA:DNA hybrids. DNase II is also called acid DNase as it hydrolyzes DNA under acidic conditions. hydrolyzes deoxyribonucleotide linkages in native and denatured DNA yielding products with 3'-phosphates. As the name implies, it is more effective at acid pHs. The protein is a ubiquitously expressed lysosomal enzyme and has been called also lysosomal DNase II
What exactly does the enzyme Dnase do to DNA?
Degrades it, breaks it down. It cleaves the phosphodiester links in the DNA's backbone.
DNAase is an enzyme that catalyzes the hydrolysis of the covalent bonds that join nucleotides together.?
The answer is in your question. The covalent bonds that join the nucleotides together would be hydrolysed. Hydrolysis is the breaking of covalent bonds by the addition of an OH to one side of the break and an H to the other side. After hydrolysis the two pieces can separate. So a DNA molecule is broken down to individual nucleotides by DNase.
Can lemon be used as an enzyme for DNA extraction?
it's an experiment. i dont have meat tenderizer, contact lens cleaner, or pineapple juice on hand. so i want to know if lemon juice can be used as an enzyme in DNA extraction. please answer ASAP
What is the co-factor for the enzyme that degrades DNA?
A cofactor is a non-protein chemical compound that is bound to a protein and is required for the protein's biological activity. Mn ++ and Mg++ are cofactors for Deoxyribonuclease I - this cleaves double-stranded or single stranded DNA. Cleavage preferentially occurs adjacent to pyrimidine (C or T) residues, and the enzyme is therefore an endonuclease. Major products are 5'-phosphorylated di, tri and tetranucleotides. In the presence of magnesium ions, DNase I hydrolyzes each strand of duplex DNA independently, generating random cleavages. In the presence of manganese ions, the enzyme cleaves both strands of DNA at approximately the same site, producing blunt ends or fragments with 1-2 base overhangs.
Why we use DNase and lysozyme in lysis step during protein purification?
The reason that DNAse is used during protein purification is in order to achieve a better purified protein solution. DNAse is a specific enzyme that degardes only DNA, leaving protein alone, thus purifying protein from DNA (genomic or otherwise). Lysozyme is an enzyme used for further purification of protein from other cellular debris. The lysozyme enzyme is commonly used to degrade, or lyse, the bacterial cell walls of both Gram positive and Gram negative bacteria. Lysozyme is also very easy to use in the lab; there is no necessary heating or shaking of the sample required for lysozyme to work efficiently. Such agitation and heating required for the use of other protein purification enzymes can cause protein denaturation, which would make your protien purification experiment much more difficult and would reduce the total amount of purified protein in your final solution. Lysozyme, also called glycosidase, is the enzyme responsible for cleaving the bond between N-acetyl muramic acid and N acetyl glucosamine. These groups are the polymers that make up the cell wall of bacteria and, thus, lysozyme cleaves the important polymers of bacterial cell walls. Lysozyme is also found in tears, saliva and phagocytic cells. The enzyme in these stats serves as an antibacterial agent, particularly against Gram positive cells. So, DNAse is used to degrade DNA, or nucleic acids and lysozyme is used to degrade polymers of monosachharides. Thus, out of the 4 major biological molecules: protein, nucleic acids, sugars, and fats, the DNAse and lysozyme enzymes degrade 2 of the 4, leaving your final solution with only protein and fat. Protein and fat can be easily purified from each other without enzymes since proteins are polar and fatty acids are nonpolar.