Reece James was one of England's best performers against Denmark at Wembley.
Gareth Southgate's side slumped to a 1-0 defeat on a disappointing evening.
But James was sent-off after the game after confronting the Spanish official.
REPORT: Harry Maguire sent-off as England lose 1-0 to Denmark on Wednesday.
Reece James was shown a red card by the referee after the full-time whistle for dissent on a tough evening for England against Denmark.
Chelsea right back James was making his first start for his country and was one of Gareth Southgate's finest performers, in a 1-0 defeat against the Danes at Wembley which saw Harry Maguire sent-off in the opening 31 minutes for two yellow cards.
But after a game where England didn't get the rub of the green from Spanish referee Jesus Gil Manzano, he displayed a red card for the second time on the night to James after the 20-year-old confronted the official following the end of the game.
Reece James was shown a red card after the full-time whistle as England lost to Denmark in the #NationsLeague pic.twitter.com/D9RTpZlMNZ
The incident after the game capped off a disappointing night for England, who failed to capitalise on their victory against Belgium on Sunday.
It means Denmark go level on points with England in their UEFA Nations League group, with the Belgians two points ahead on a total of nine.
James though, making his third cap for England, was lively out-wide on the right with his delivery threatening throughout.
But the 20-year-old was punished for dissent at the end and now faces at least a one-match suspension, meaning he will be unavailable for the trip to Belgium in November.
The reaction on social media to James' red card was one of anger directed at the official, with one saying: 'Reece James just said to that ref what everyone in England was thinking.'
Another similarly tweeted: 'Reece James telling the ref what he though of that refereeing performance is what football needs. Lad.'
Ex-England international Jamie Redknapp said of the decision afterwards: 'It’s a massive shame because I thought he [James] was sensational today.
'Emotions perhaps got the better of him. I thought the referee was useless today. If he has sworn at him then there’s nothing he can do he’s got to be sent off.'
Manager Gareth Southgate, speaking to Sky Sports, added: 'The referee says he has sent him off for dissent. That is a lesson he has got to learn as a player. He has had an incredible week.
'The quality of play down the right, he had a fantastic game. It’s something that shouldn’t have happened but in the overall week has had made a positive impression.'
Alex Scott, a former England international, said of James' overall performance: 'Some of the balls into the box were great, I thought Reece James was England’s best player today.'