Barcelona are facing a hectic period of four matches across the next 11 days.
That run includes El Clasico and a Champions League game against Juventus.
First up is Getafe in La Liga and boss Ronald Koeman 'is ready to make changes'.
It is believed Miralem Pjanic, Sergino Dest and Trincao will all face Getafe.
Barcelona manager Ronald Koeman is ready to shake up his side with a number of changes to face Getafe this weekend, according to reports in Spain.
Koeman is preparing to oversee a frantic four games in 11 days and it is vital he uses the depth of his squad, starting away to Getafe in La Liga on Saturday.
Wednesday's front page of newspaper Sport details that Miralem Pjanic, who arrived in the summer from Juventus in a £54million move, Trincao and Sergino Dest, the newest recruit from Ajax, are all ready to start at the Coliseum Alfonso Perez.
Barcelona's packed schedule includes El Clasico at home to Real Madrid on October 24, sandwiched between two Champions League games.
Koeman's side face Hungarian side Ferencvaros in their Group G opener on October 20 before facing Cristiano Ronaldo's Juventus on October 28.
Pjanic, Trincao and Dest are yet to start any of Barcelona's opening three games, having been handed fleeting roles off the bench.
The international break has complicated Koeman's preparations for the visit to Getafe with a number of players due back late on Thursday.
That leaves just two training sessions to ensure there is no fatigue and players are clued up on the tactical plan for the opposition.
His South American stars, including Philippe Coutinho and Lionel Messi, could even be given an extra day's rest after such long travel commitments for their countries.
They sit fifth in La Liga after three games played with two wins - against Villarreal and Celta Vigo - and a draw with Sevilla.
Koeman has persisted with a 4-2-3-1 formation, spearheaded by Messi, in a bid to maximise Coutinho in a No 10 role.
The club typically adopted a 4-3-3 from youth teams to the first team but Koeman has stuck to his guns and has trusted the same personnel more or less in his first three games with Coutinho the pick of the bunch.
The Brazilian has been thriving and flanked by Ansu Fati and Antoine Griezmann, Koeman has created an exciting and dynamic front four as he looks to wrestle back the title from rivals Real Madrid.